Préparation et calibration d'un trieur au sein du laboratoire P3 de l'IGH

Preparation and calibration of a sorter in the P3 laboratory at the Institute of Human Genetics (IGH) to ensure that sorting takes place in optimum conditions. Here, the deflectors that transmit the sorting orders are being cleaned. The cells (healthy and infected) in a blood sample from a patient infected with HIV-1 are marked and prepared for this sorter (homogenisation and filtration). It is in this laboratory that a marker has been identified that makes it possible to differentiate between dormant or reservoir cells, infected with HIV, and healthy cells. This discovery will make it possible to isolate and analyse these reservoir cells which, by silently hosting the virus, are responsible for its persistence even in patients receiving antiretroviral treatment, whose viral load is undetectable. It offers new therapeutic strategies for targeting infected cells.