Montage de coupes de cerveaux de souris sur des lames, après marquage par immunohistochimie

Mounting brain sections on slides after labelling via immunohistochemistry (IHC), for viewing through a microscope. These sections have been immersed in baths containing antibodies that make it possible, in the experiment, to locate doublecortin proteins, which are markers of immature neurons. The study focuses on adult neurogenesis, i.e. the development of neurons during adulthood, in the hippocampus. The sections are then immersed in a bath that allows the detection of proteins via fluorescence or visible light. The brains used are from transgenic mice that express a protein associated with Alzheimer’s disease. The aim of these experiments is to intervene in the neurogenesis process to improve the memory of patients affected by neurodegenerative diseases.