Marquage par immunohistochimie de coupes de cerveaux de souris

Labelling mouse brain sections via immunohistochemistry (IHC). The baths in which these sections are immersed contain antibodies that make it possible, in the experiment, to locate doublecortin proteins, which are markers of immature neurons. The study focuses on adult neurogenesis, i.e. the development of neurons during adulthood, in the hippocampus. The sections are then placed in baths to wash them, and the proteins are detected via fluorescence or visible light. The brains used are from transgenic mice that express a protein associated with Alzheimer’s disease. The aim of these experiments is to intervene in the neurogenesis process to improve the memory of patients affected by neurodegenerative diseases.