© Jean-Christophe AME / BSC / UNISTRA / CNRS Images
Reference
20170099_0005
Coupures d’ADN HeLa produites par un laser dans des cellules cancéreuses
Co-marking (in yellow) DNA breaks in HeLa cells, observed using fluorescence microscopy. DNA is marked in blue by the DAPI protein, which is specked with green as a result of introducing phosphorylated RPA protein, which bonds to single-stranded DNA. The red marking is attributable to bromodeoxyuridine (BrdU - a molecule used to determine a cell's division cycle phase), which bonds to the breaks. The aim is to study post-break DNA resynthesis. The yellow areas are the result of the superimposition of the aforementioned colours.
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