Réparation de cassures d'ADN dans des cellules cancéreuses HeLa
HeLa cells in apoptosis, or cell death, observed using fluorescence microscopy. DNA is marked in blue, histone H3 in green, and cytochrome C (released by mitochondria upon cell death) in red. Here, researchers are studying a particular case of cell death, known as mitotic catastrophe. This phenomenon may be caused by a defect in the cell cycle sequence or by a DNA alteration. More specifically, the scientists are interested in cells that have lost the PARG function. This enzyme - Poly (ADP-ribose) glycohydrolase (PARG) - degrades poly(ADP-ribose), a small branched molecule that temporarily modifies proteins with a role in repairing damage to DNA.