20170086_0001
© Bénédicte Charrier / LBI2M / CNRS Images

Thalle de l'algue brune filamenteuse "Ectocarpus siliculosus"

Reference

20170086_0001

Production year

2014

Max. size

20.59 x 21.34 cm / 300 dpi

Caption

Thallus of the filamentous brown alga Ectocarpus siliculosus, measuring 2 mm in diameter, floating on the surface of a droplet of seawater, observed using a bright-field microscope. The Ectocarpus alga is used as a plant model, as it is easy to cultivate in the laboratory in small spaces, such as Petri dishes. It also has a short reproductive cycle of around six weeks. Its genome was the first brown alga genome to be sequenced. It has a simple morphology: the cells that make up its filaments are all accessible for observation and experimentation. As a result, research scientists are trying to identify the factors that control its morphogenesis (the biological process that generates the shape of an organism). To do this, they are trying to acquire several types of biological data: the biomechanical parameters of its surface (cell wall) using atomic force microscopy (AFM); the cell-dependent expression profile using laser capture microdissection (LCM) followed by RNA-Seq sequencing; and its growth dynamic using time-lapse microscopy and by labelling cell constituents such as the cytoskeleton, the wall and vesicular traffic. The results show that it is possible to simulate these processes by taking into account solely biophysical criteria, such as the internal pressure of cells, their shape, their rigidity and the thickness of their wall. In addition, an original apical growth strategy different to that used by other eukaryotic organisms studied has been observed in Ectocarpus filaments.

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