Triple marquage d’un neurone d’hippocampe de rat

Triple marking of a rat hippocampus neuron, observed using full-field fluorescence imaging. In green, the surface marking of the GluA2 sub-unit of the AMPA-type receptors activated by glutamate. The clathrin, marked in red, enables identification of the endocytic pits that are the receptors’ transport channel to the inside of the cell. The GluA2 internalised sub-unit of the AMPA-type receptors activated by glutamate is marked in blue. A triple co-location of the clathrin, surface and intracellular receptors is revealed in way of the synapses, as is the accumulation of intracellular receptors in way of the cell body.

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