See Photo report
© Cyril FRESILLON/IPBS/CNRS Images
Sampling a small slice of human dermal tissue from a 24-well plate
Max. size41.72 x 27.77 cm / 300 dpi
Add to my wishlist
The use of media visible on the CNRS Images Platform can be granted on request. Any reproduction or representation is forbidden without prior authorization from CNRS Images (except for resources under Creative Commons license).
No modification of an image may be made without the prior consent of CNRS Images.
No use of an image for advertising purposes or distribution to a third party may be made without the prior agreement of CNRS Images.
For more information, please consult our general conditions
Sampling a small slice of human dermal tissue from a 24-well plate. This tissue is a dermal substitute engineered from cells taken during a skin biopsy. A pulsed electric field was applied to this tissue to help a GFP-expressing plasmid (Green Fluorescent Protein, a protein that emits green fluorescent light) penetrate its cells. After 24 or 48 hours, the tissue was examined by multiphoton microscope in order to locate and quantify the amount of GFP-expressing cells. This experiment enabled us to obtain in vitro rates of transfection comparable to those obtained in vivo. It thus proved possible to create a substitute for human skin in vitro, i.e. a dermal model that can be used in the laboratory. This will allow us to limit the number of in vivo tests on animal models.