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Withdrawal of subcutaneously induced murine fibrosarcoma sections dipped for smoothing in a 37°C bath. These sections were paraffin-embedded. They were then deposited on glass slides. The fibrosarcoma models spontaneously generated HEV blood vessels (high endothelium veinules) 8 days after induction in the mouse. Researchers were seeking to detect the presence of specific RNA for these blood vessels on the glass slides. They thus co-marked this RNA using specific proteins for these vessels.
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