Transfer of the paraffin-embedded tumour sections for smoothing to a 37°C bath

After the microtome sectioning of murine fibrosarcoma induced subcutaneously in mice, the paraffin-embedded tumour sections were transferred for smoothing to a 37°C bath. They were then deposited on glass slides. The fibrosarcoma models spontaneously generated HEV blood vessels (high endothelium veinules) 8 days after induction in the mouse. Researchers were seeking to detect the presence of specific RNA for these blood vessels on the glass slides. They thus co-marked this RNA using specific proteins for these vessels.