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Yann Kerdiles, médaille de bronze du CNRS 2018. Chercheur en immunologie, spécialisé dans le domaine des cellules lymphoïdes innées du groupe 1 : les cellules Natural Killer et les ILC1, au Centre d’immunologie de Marseille-Luminy.

Cellules de fibroblastes rénaux de singe vert africain COS-7 observées en microscopie confocale à fluorescence super-résolution dSTORM (direct stochastic optical reconstruction microscopy). Les cellules sont marquées avec la phalloïdine conjuguée à Alexa Fluor 647. On observe ainsi les fibres de stress et les filaments d'actine avec une résolution de 20 nm. La technique de super résolution, ici le dSTORM permet d’observer des structures par fluorescence de plus en plus finement. Ici, les…

A 20 µm thick cross-section of a seminiferous tubule in a mouse testicle, observed using multicolour confocal microscopy. The cell nuclei are visible in yellow (DAPI). Testicular macrophages are mobilised to defend sperm cells. By releasing specific molecules, these guardians of fertility prevent other immune system agents from entering the testes. A recent study described two types of testicular macrophage, establishing the origin, development and characteristics of these immune cells. The…

A 20 µm thick cross-section of a seminiferous tubule in a mouse testicle, observed using multicolour confocal microscopy. The heads of the sperm cells have been marked in red by immunofluorescence (phalloidin marked with the fluorochrome Alexa-647), and their tails are shown in blue (DAPI). Testicular macrophages are mobilised to defend sperm cells. By releasing specific molecules, these guardians of fertility prevent other immune system agents from entering the testes. A recent study described…

Montage optique combinant microscope à déplétion par émission stimulée (STED) et spectroscopie de corrélation de fluorescence (FCS) développé au sein de la plateforme ImagImm. Ce microscope STED fait partie des microscopes dits à haute résolution et son couplage avec la FCS permet de sonder avec précision la dynamique de molécules au sein de cellules vivantes. Les chercheurs explorent le lien entre organisation de la membrane plasmique et signalisation.

Montage optique de spectroscopie croisée de corrélation de fluorescence (FCCS : Fluorescence Cross-Correlation Spectroscopy) où l’on devine des lentilles, des diaphragmes et des miroirs dichroïques. Il a été développé au sein de la plateforme ImagImm. La microscopie actuelle repose largement sur l’emploi de lasers de différentes longueurs d’onde ("couleurs") pour l’excitation de molécules fluorescentes. Le travail des ingénieurs en charge de cette technologie repose sur la métrologie des…

A 20 µm thick cross-section of a seminiferous tubule in a mouse testicle, observed using multicolour confocal microscopy. The heads of the sperm cells have been marked in red by immunofluorescence (phalloidin marked with the fluorochrome Alexa-647), and their tails are shown in cyan (DAPI). Testicular macrophages are mobilised to defend sperm cells. By releasing specific molecules, these guardians of fertility prevent other immune system agents from entering the testes. A recent study described…

Subpopulation of immune cells from the small intestine, observed using 10-colour spectral imaging. The image was generated after immunofluorescence labelling and by using a combination of several lasers, including one white laser providing a wide range of wavelengths, i.e. colours. A high-magnification, oil-immersed lens was used. In confocal microscopy, the sample is scanned at high speed by laser beams, point by point. This technique produces fine optical sections (at different levels in a…

Section of a mouse kidney observed using a multicolour confocal microscope. This microscope image, which has a resolution of around 200 nanometres, was obtained using immunofluorescence labelling: AF488-WGA in green, AF568 phalloidin in red and DAPI in blue. The image was generated using a combination of several lasers, including one white laser providing a wide range of wavelengths (colours). In confocal microscopy, the sample is scanned by laser beams, point by point. This technique produces…

Section of a seminiferous tubule from a mouse testis measuring 20 µm thick, observed using a multicolour confocal microscope. The heads of the spermatozoa have been labelled in red using immunofluorescence (phalloidin labelled with the fluorochrome Alexa-647), while their tails are shown in cyan (DAPI). Testicular macrophages are mobilised to defend the spermatozoa. By emitting specific molecules, these fertility guardians prevent other elements in the immune system entering the testes. A…

Section of a seminiferous tubule from a mouse testis measuring 20 µm thick, observed using a multicolour confocal microscope. The cell nuclei are visible in cyan (DAPI), with the actin, which reveals the cell skeleton, in red (phalloidin labelled with the fluorochrome Alexa-647) and the macrophages in green Testicular macrophages are mobilised to defend the spermatozoa. By emitting specific molecules, these fertility guardians prevent other elements in the immune system entering the testes. A…

Section of a seminiferous tubule from a mouse testis measuring 20 µm thick, observed using a multicolour confocal microscope. The cell nuclei are visible in cyan (DAPI), with the actin, which reveals the cell skeleton, in yellow (phalloidin labelled with the fluorochrome Alexa-647). Testicular macrophages are mobilised to defend the spermatozoa. By emitting specific molecules, these fertility guardians prevent other elements in the immune system entering the testes. A recent study made it…

Section of a seminiferous tubule from a mouse testis measuring 20 µm thick, observed using a multicolour confocal microscope. The stem cells that will produce the spermatozoa can be seen from the periphery to the lumen of the tubule. The spermatozoa can be distinguished by their tails. The cell nuclei are visible in red (DAPI). Testicular macrophages are mobilised to defend the spermatozoa. By emitting specific molecules, these fertility guardians prevent other elements in the immune system…

A 20 µm thick cross-section of a seminiferous tubule in a mouse testicle, observed using multicolour confocal microscopy. The cell nuclei are visible in yellow (DAPI). Testicular macrophages are mobilised to defend sperm cells. By releasing specific molecules, these guardians of fertility prevent other immune system agents from entering the testes. A recent study described two types of testicular macrophage, establishing the origin, development and characteristics of these immune cells. The…

Coupe d’un testicule de nouveau-né de souris d'une épaisseur de 20µm, en microscopie confocale multi-couleur. Les noyaux cellulaires sont visibles en rose (DAPI). Les macrophages testiculaires sont mobilisés pour défendre les spermatozoïdes. En émettant des molécules spécifiques, ces gardiens de la fertilité empêchent d'autres acteurs du système immunitaire de pénétrer dans les testicules. Une étude a permis de caractériser deux types de macrophages testiculaires et d'établir l'origine, le…