Analyse de génotypage du maïs : extraction d’ADN foliaire

Maize genotype analyses: leaf DNA extraction. Plant matter is first ground in a lysis buffer and then separated in a centrifuge. Leaf waste, including cell walls, is denser, collecting at the bottom. The supernatant will contain DNA, mixed with carbohydrates, proteins and pigments. Additional centrifuging will separate these components according to their respective densities, in order to obtain and subsequently analyse the pure DNA fraction. Scientists at the Quantitative Genetics and Evolution unit in Le Moulon are studying the genetic, epigenetic and molecular control of qualitative and quantitative characteristics and their importance in the adaptation and evolutionary history of plant species. They optimise selection processes based on target characteristics (such as growth, development and adaptation), taking advantage of breakthroughs in genomics and genetic resources, and allowing for changes in environmental conditions and farming methods.