Calibration d'un trieur au sein du laboratoire P3 de l'institut de génétique humaine (IGH)

Calibration of a sorter in the P3 laboratory at the Institute of Human Genetics (IGH) before the introduction of cells (healthy and infected) from a blood sample from a patient infected with HIV-1. This sorter works using FACS (fluorescence-activated cell sorting) and makes it possible to collect cell populations that meet precise criteria in sterile conditions suitable for culturing. The cells are sorted depending on combinations of predefined markers. It is in this laboratory that a marker has been identified that makes it possible to differentiate between dormant or reservoir cells, infected with HIV, and healthy cells. This discovery will make it possible to isolate and analyse these reservoir cells which, by silently hosting the virus, are responsible for its persistence even in patients receiving antiretroviral treatment, whose viral load is undetectable. It offers new therapeutic strategies for targeting infected cells.